Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Gas chromatography-mass spectrometric analysis of nematicidal essential oil of Valeriana amurensis P Smirn ex Kom (Valerianaceae) roots and its activity against Heterodera avenae

Yun Chao Li, Hua Ji, Hong Tao Tao Li

Institute of Genetics and Physiology, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050051, PR China;

For correspondence:- Hong Tao Li Email: lihongtao1314@hotmail.com Tel:+8631113933107622

Received: 25 March 2015 Accepted: 25 July 2015 Published: 29 September 2015

Citation: Li YC, Ji H, Li HT. Gas chromatography-mass spectrometric analysis of nematicidal essential oil of Valeriana amurensis P Smirn ex Kom (Valerianaceae) roots and its activity against Heterodera avenae. Trop J Pharm Res 2015; 14(9):1673-1678 doi: 10.4314/tjpr.v14i9.18

© 2015 The authors.
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Abstract

Purpose: To investigate the chemical composition and nematicidal activity of the essential oil of Valeriana amurensis roots against cereal cyst nematodes (Heterodera avenae).

Methods: The essential oil of V. amurensis roots was obtained by hydrodistillation and analyzed by gas chromatography (GC) and gas chromaotography-mass spectrometry (GC-MS). The nematicidal activity of the essential oil and its major constituents was determined against second stage juveniles of H. avenae.

Results: A total of 33 components of the essential oil were identified. The major constituents were bornyl acetate (12.5 %), patchoulol (11.6 %), caryophyllene (8.2 %), 3-methylvaleric acid (7.3 %) and isovaleric acid (6.5 %). The essential oil exhibited nematicidal activity against H. avenae with a medium lethal concentration (LC₅₀) value of 311.6 µg/mL. The major constituents, isovaleric acid and 3-methylvaleric acid, exhibited nematicidal activity against H. avenae with LC₅₀ of 218.2 and 683.8 µg/mL, respectively.

Conclusion: The study indicates that the essential oil of V. amurensis roots and its two major constituents, isovaleric acid and 3-methylvaleric acid, have a potential to be developed to natural nematicides for the control of cereal cyst nematodes.

Keywords: Valeriana amurensis, Heterodera avenae, Nematicidal activity, Isovaleric acid, 3-Methylvaleric acid, Essential oil, Cereal cyst nematodes